LOCUS       40924_22799        7114 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Moss transformation vector pGUS-NPTII-2 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7114)
  AUTHORS   Sakakibara K, Nishiyama T, Sumikawa N, Kofuji R, Murata T, Hasebe M.
  TITLE     Involvement of auxin and a homeodomain-leucine zipper I gene in 
            rhizoid development of the moss Physcomitrella patens
  JOURNAL   Development 130 (20), 4835-4846 (2003)
  PUBMED    12917289
REFERENCE   2  (bases 1 to 7114)
  AUTHORS   Hiwatashi Y, Hasebe M.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-FEB-2012) Contact:Yuji Hiwatashi National Institute 
            for Basic Biology, Evolutionary Biology; Nishigounaka 38, Myoudaiji,
            Okazaki, Aichi 4448585, Japan
REFERENCE   3  (bases 1 to 7114)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7114)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development"; date: "2003"; volume: "130"; issue: "20"; pages: 
            "4835-4846"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (03-FEB-2012) Contact:Yuji Hiwatashi National Institute for Basic 
            Biology, Evolutionary Biology; Nishigounaka 38, Myoudaiji, Okazaki, 
            Aichi 4448585, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7114
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             58..1866
                     /codon_start=1
                     /label=GUS
                     /note="beta-glucuronidase"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAI
                     AVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEV
                     MEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHD
                     FFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQ
                     VVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGEQF
                     LINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDW
                     ADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARD
                     KNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISD
                     LFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMY
                     TDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRK
                     PKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     terminator      1940..2192
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        2277..2622
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             2652..3443
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPXELFAXLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    3516..3692
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     primer_bind     complement(4221..4237)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(4270..4288)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4298..4314)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      4456..4911
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4937..5041
                     /label=AmpR promoter
     CDS             5042..5899
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6073..6661
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    6949..6970
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        6985..7015
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7023..7039
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7047..7063
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        7084..7102
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ggtaccgggc cccccctcga ggtcgactct agaggatccc cgggtggtca gtcccttatg
       61 ttacgtcctg tagaaacccc aacccgtgaa atcaaaaaac tcgacggcct gtgggcattc
      121 agtctggatc gcgaaaactg tggaattgat cagcgttggt gggaaagcgc gttacaagaa
      181 agccgggcaa ttgctgtgcc aggcagtttt aacgatcagt tcgccgatgc agatattcgt
      241 aattatgcgg gcaacgtctg gtatcagcgc gaagtcttta taccgaaagg ttgggcaggc
      301 cagcgtatcg tgctgcgttt cgatgcggtc actcattacg gcaaagtgtg ggtcaataat
      361 caggaagtga tggagcatca gggcggctat acgccatttg aagccgatgt cacgccgtat
      421 gttattgccg ggaaaagtgt acgtatcacc gtttgtgtga acaacgaact gaactggcag
      481 actatcccgc cgggaatggt gattaccgac gaaaacggca agaaaaagca gtcttacttc
      541 catgatttct ttaactatgc cggaatccat cgcagcgtaa tgctctacac cacgccgaac
      601 acctgggtgg acgatatcac cgtggtgacg catgtcgcgc aagactgtaa ccacgcgtct
      661 gttgactggc aggtggtggc caatggtgat gtcagcgttg aactgcgtga tgcggatcaa
      721 caggtggttg caactggaca aggcactagc gggactttgc aagtggtgaa tccgcacctc
      781 tggcaaccgg gtgaaggtta tctctatgaa ctgtgcgtca cagccaaaag ccagacagag
      841 tgtgatatct acccgcttcg cgtcggcatc cggtcagtgg cagtgaaggg cgaacagttc
      901 ctgattaacc acaaaccgtt ctactttact ggctttggtc gtcatgaaga tgcggacttg
      961 cgtggcaaag gattcgataa cgtgctgatg gtgcacgacc acgcattaat ggactggatt
     1021 ggggccaact cctaccgtac ctcgcattac ccttacgctg aagagatgct cgactgggca
     1081 gatgaacatg gcatcgtggt gattgatgaa actgctgctg tcggctttaa cctctcttta
     1141 ggcattggtt tcgaagcggg caacaagccg aaagaactgt acagcgaaga ggcagtcaac
     1201 ggggaaactc agcaagcgca cttacaggcg attaaagagc tgatagcgcg tgacaaaaac
     1261 cacccaagcg tggtgatgtg gagtattgcc aacgaaccgg atacccgtcc gcaaggtgca
     1321 cgggaatatt tcgcgccact ggcggaagca acgcgtaaac tcgacccgac gcgtccgatc
     1381 acctgcgtca atgtaatgtt ctgcgacgct cacaccgata ccatcagcga tctctttgat
     1441 gtgctgtgcc tgaaccgtta ttacggatgg tatgtccaaa gcggcgattt ggaaacggca
     1501 gagaaggtac tggaaaaaga acttctggcc tggcaggaga aactgcatca gccgattatc
     1561 atcaccgaat acggcgtgga tacgttagcc gggctgcact caatgtacac cgacatgtgg
     1621 agtgaagagt atcagtgtgc atggctggat atgtatcacc gcgtctttga tcgcgtcagc
     1681 gccgtcgtcg gtgaacaggt atggaatttc gccgattttg cgacctcgca aggcatattg
     1741 cgcgttggcg gtaacaagaa agggatcttc actcgcgacc gcaaaccgaa gtcggcggct
     1801 tttctgctgc aaaaacgctg gactggcatg aacttcggtg aaaaaccgca gcagggaggc
     1861 aaacaatgaa tcaacaactc tcctggcgca ccatcgtcgg ctacagcctc gggaattgct
     1921 accgagctcg aatttccccg atcgttcaaa catttggcaa taaagtttct taagattgaa
     1981 tcctgttgcc ggtcttgcga tgattatcat ataatttctg ttgaattacg ttaagcatgt
     2041 aataattaac atgtaatgca tgacgttatt tatgagatgg gtttttatga ttagagtccc
     2101 gcaattatac atttaatacg cgatagaaaa caaaatatag cgcgcaaact aggataaatt
     2161 atcgcgcgcg gtgtcatcta tgttactaga tcgggaattc tgcagcccat cgaattcctg
     2221 cagcccccct actccaaaaa tgtcaaagat acagtctcag aagaccaaag ggctattgag
     2281 acttttcaac aaagggtaat ttcgggaaac ctcctcggat tccattgccc agctatctgt
     2341 cacttcatcg aaaggacagt agaaaaggaa ggtggctcct acaaatgcca tcattgcgat
     2401 aaaggaaagg ctatcattca agatgcctct gccgacagtg gtcccaaaga tggaccccca
     2461 cccacgagga gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa gcaagtggat
     2521 tgatgtgaca tctccactga cgtaagggat gacgcacaat cccactatcc ttcgcaagac
     2581 ccttcctcta tataaggaag ttcatttcat ttggagagga cagcccaagc atggagaacc
     2641 gacctgcagg catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg
     2701 agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt
     2761 tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc
     2821 tgaatgaact ccaagacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt
     2881 gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag
     2941 tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg
     3001 ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag
     3061 cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg
     3121 atctggacga agagcatcag gggctcgcgc cakccgaact gttcgccang ctcaaggcgc
     3181 ggatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca
     3241 tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc
     3301 gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg
     3361 ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct
     3421 atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcggacggt acgctgaaat
     3481 caccagtctc tctctacaaa tctatctctc tctattttct ccataaataa tgtgtgagta
     3541 gtttcccgat aagggaaatt agggttctta tagggtttcg ctcatgtgtt gagcatataa
     3601 gaaaccctta gtatgtattt gtatttgtaa aatacttcta tcaataaaat ttctaattcc
     3661 taaaaccaaa atccagtact aaaatccaga tctcctaaag tccctataga tctttgtcgt
     3721 gaatataaac cagacacgag acgactaaac ctggagccca gacgccgttc gaagctagaa
     3781 gtaccgctta ggcaggaggc cgttagggaa aagatgctaa ggcagggttg gttacgttga
     3841 ctcccccgta ggtttggttt aaatatgatg aagtggacgg aaggaaggag gaagacaagg
     3901 aaggataagg ttgcaggccc tgtgcaaggt aagaagatgg aaatttgata gaggtacgct
     3961 actatactta tactatacgc taagggaatg cttgtattta taccctatac cccctaataa
     4021 ccccttatca atttaagaaa taatccgcat aagcccccgc ttaaaaattg gtatcagagc
     4081 catgaatagg tctatgacca aaactcaaga ggataaaacc tcaccaaaat acgaaagagt
     4141 tcttaactct aaagataaaa gatctttcaa gatcaaaact agttccctca caccggtgac
     4201 ggggatcgca tgcgatgggg gatccactag ttctagagcg gccgccaccg cggtggagct
     4261 ccaattcgcc ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc
     4321 gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg
     4381 ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc
     4441 tgaatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     4501 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     4561 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     4621 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
     4681 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     4741 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
     4801 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     4861 tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt taggtggcac
     4921 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     4981 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     5041 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     5101 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     5161 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     5221 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     5281 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     5341 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     5401 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     5461 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     5521 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     5581 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     5641 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     5701 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     5761 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     5821 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     5881 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     5941 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     6001 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     6061 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     6121 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     6181 ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt
     6241 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     6301 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     6361 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     6421 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     6481 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     6541 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     6601 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     6661 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     6721 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     6781 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     6841 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
     6901 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
     6961 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
     7021 aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagcg
     7081 cgcaattaac cctcactaaa gggaacaaaa gctg
//
